We explore vitrification, a method that uses high concentrations of chemicals called cryoprotective agents (CPAs) to preserve organs at very low temperatures without ice formation. While this method could theoretically keep organs viable indefinitely, the high CPA concentrations needed can be toxic to tissues. Our study tested three CPAs—VM3, M22-PVP and M22—on rat kidney slices to assess their toxicity. We found that VM3 was the least toxic, followed by M22-PVP and M22. These findings highlight the importance of selecting CPAs that are less toxic for organ preservation. Our results, combined with future studies on how temperature affects CPA toxicity, will help develop better methods for long-term kidney storage. This could significantly improve organ transplantation by increasing available organs, enhancing donor-recipient matching and promoting fair access, ultimately improving patient outcomes.