This study developed a method for the assay of respiration in free swimming Caenorhabditis elegans (C. elegans). The technique employed direct monitoring of acidification of medium by these nematodes. Samples which might affect respiration were examined, including sugars and Escherichia coli (E. coli), the laboratory food source for C. elegans. The bacteria stimulated respiration but the sugars did not. The decrease in fluid environment pH is blocked by sodium azide, a known mitochondrial inhibitor. This monitoring was achieved using pH probes.
Cowpea aphids (Aphis craccivora) are a major pest of cowpeas (Vigna unguiculata), which feed on the plant at the vegetative stage by sucking the sap. In an endeavour to find an new method of controlling aphid infestation in the Northern Ghana, an in vitro study was carried out to investigate the effect of different concentrations of aqueous false yam (Icacina oliviformis) tuber extract against cowpea black aphids (Aphis craccivora Koch).
Pseudomonas aeruginosa is the leading cause of ocular infections in those who wear contact lenses. Others have previously done a study using the antioxidant selenium-coated contact lenses to inhibit the bacteria in an animal model. However, selenium is very toxic even in small quantities. In this study, green tea which is known for its antioxidant property was used to treat contact lenses.
With the growing number of antibiotic-resistant pathogens, there is a need for new antibiotics. Bacteria within the order Actinomycetales produce the majority of known antibiotic compounds but harbor cryptic secondary metabolic pathways that likely produce thousands more antibiotics awaiting discovery. This has recently renewed interests in bioprospecting for novel Actinomycetales in underexplored environments, such as the lower atmosphere, and activating cryptic secondary metabolic pathways in previously characterized members of this order.
Understanding bacterial translation is of interest to many high school and college biology students, but hands-on research of this process has traditionally been inaccessible because of the expertise required for molecular cloning. There is also a need for more and better translational control elements that can be used for genetic circuits with applications in medicine, biotechnology, bioremediation, and biomaterials.