Mycobacterium tuberculosis causes epidemic levels of tuberculosis each year and more strains of the disease are becoming resistant to the medications used to treat it. Alanine racemase is a convenient target for new drug candidates for tuberculosis as this enzyme is not found in human cells and in M. tuberculosis is only active when in the dimer form. To elucidate the dimerization process, the equilibrium association constants for monomer-dimer equilibrium were determined using high-performance size-exclusion liquid chromatography in a variety of mobile phases.
Recovery following stroke is dependent on several factors, including neuroplasticity. Dietary supplementation with folic acid impacts neuroplasticity. Folic acid is a B-vitamin and is a major component of one-carbon metabolism. The aim of this study is to determine the impact of one-carbon supplementation on neuroplasticity after ischemic stroke using a mouse model.