The objective of this research was to test the in vitro anti-angiogenic activity of auraptene, a coumarin from citrus peel oil. We tested the ability of auraptene to inhibit in vitro angiogenesis using vascular endothelial growth factor (VEGF)-induced human umbilical endothelial cells (HUVEC) growth stimulation.
The metabolic process of glutamate synthesis in Saccharomyces cerevisiae is governed by a functional model that assumes extramitochondrial localization of glutamate dehydrogenase (GDH). In S. cerevisiae, GDH exists as three isozymes: Gdh1p and Gdh3p, which are NADP-dependent, and Gdh2p, which is NAD-dependent. Previous work has suggested nuclear and/or cytosolic localization, but definitive data are lacking. We attempted to determine the subcellular localization of the three GDH isozymes by enzymatic activity.