Use of iLAP Plates as a New Rapid Screening Method for the Evaluation of Various Human Recombinant Coproporphyrinogen Oxidase Mutants with Wider Possible Applications

The amino acid sequence of the enzyme coproporphyrinogen oxidase (copro'gen oxidase) in the heme biosynthetic pathway is known but its catalytic mechanism has yet to be determined. As a result, it is essential to carry out site-directed mutagenesis on highly conserved amino acids to help determine which amino acids are essential for catalysis. Often, a large number of mutants are created in this fashion, so it is in the researcher's best interest to have a technique to evaluate such mutants efficiently, quickly, and cheaply.