Rotenone is a broad-spectrum pesticide and piscicide that inhibits the transport of electrons in NADH-Q-oxidoreductase (complex I), thereby inhibiting cellular respiration. For the past two decades, epidemiologists have suspected that exposure to rotenone increases the risk of developing Parkinsonism. Although restricted for food use in the United States, rotenone is still used in countries exporting produce. Research has determined rotenone’s presence in produce as well as processed food products, such as tea and olive oil, raising the concern that it does not degrade before and during food processing. Furthermore, there are documented issues with the organic certification of imported produce into the United States, including soybeans. Organic and non-organic national brands of soy milk were purchased from a supermarket in the Greater Cleveland Area, and two samples were analyzed in triplicate for rotenone using high-performance liquid chromatography equipped with an ultraviolet light detector (HPLC-UV). A positive determination would indicate that rotenone is not degraded during the process that turns soybeans into milk. The samples were analyzed and compared to samples intentionally spiked with rotenone before they were filtered and dissolved in acetonitrile in preparation for the detection by HPLC- UV. Rotenone was not detected in these samples. However, a reliable method was developed for filtering soy milk and analyzing it for rotenone residues. The method detection limit allows for the determination of rotenone residues in soybeans beyond the maximal residual limit fixed by the European Union.