Author: Rachel M. McNeill, William M. DeFoor, Carlos C. Goller, and Laura E. Ott
Delftia spp. are rod-shaped, gram-negative water and soil bacteria that are resistant to many commonly used antibiotics. Clinical case reports have described infections of Delftia spp. in patients with intravenous catheters, prompting us to investigate the immune response to Delftia spp. stimulation. We hypothesized that stimulation of THP-1 monocytes with Delftia spp. would elicit a pro-inflammatory response. THP-1 cells were stimulated for 24hr with two strains of heat-killed Delftia spp.: Delftia acidovorans ATCC 13751 at a multiplicity of infection (MOI) of 0.6 and 6.0, and Delftia sp. Cs1-4 MOI of 0.8 and 8.0. Purified lipopolysaccharide (LPS) was used as the positive control. Stimulation of monocytes with Delftia spp. resulted in increased monocyte mortality.