This study developed a method for the assay of respiration in free swimming Caenorhabditis elegans (C. elegans). The technique employed direct monitoring of acidification of medium by these nematodes. Samples which might affect respiration were examined, including sugars and Escherichia coli (E. coli), the laboratory food source for C. elegans. The bacteria stimulated respiration but the sugars did not. The decrease in fluid environment pH is blocked by sodium azide, a known mitochondrial inhibitor. This monitoring was achieved using pH probes.
Dr. Jason Samarasena is an interventional gastroenterologist at the University of California, Irvine (UCI) Medical Center’s Chao Comprehensive Digestive Disease Center. He specializes in the diagnosis and treatment of digestive diseases, including gastroesophageal reflux disease (GERD), pancreatitis, and biliary tract disorders through the use of state-of-the-art endoscopic technology.
With only two females left, the Northern White Rhinoceros (NWR) is nearly extinct, but a revolution in fertility treatments may pave the way to the species’ resurrection. The two surviving NWRs live in a Kenyan nature reserve, where armed guards protect them around the clock. They once roamed the planes of Uganda, Southern Chad, and South Western Sudan, but political unrest led to a growing demand for rhino horn, which in turn led to an increase in poaching in these countries.
CRISPR-Cas9 gene editing (CRISPR) has become a renowned genome editing tool that has greatly enhanced scientific research but has also caused much controversy and concern over its potential applications. With the potential of becoming a standard medical tool to fix incurable genetic diseases, the safety and efficiency of CRISPR needs to be extensively investigated to ensure that only the desired DNA modifications are made. Recent studies published in Nature have discovered how editing efficiency in cells can be influenced by the DNA damage response pathway 1,2 and are raising concerns after finding that CRISPR cuts can result in larger unwanted DNA rearrangements than previously thought 3.