Gut Feeling, Gut Thinking

Microbes in the human body outnumber human cells at approximately a 10:1 ratio. There are 100 trillion microbes living in the intestines alone; these microscopic communities can account for as much as 6 pounds of a person’s body weight[1]. Gut microbes have been known to promote healthy digestion, but recent research uncovered these microbes’ influence on the body’s immune and nervous system, as well as potential implications in therapies for patients with Autism Spectrum Disorder (ASD).     

The Biology of Native and Adapted CRISPR-Cas Systems

Clustered regularly interspaced short palindromic repeats (CRISPR) systems have revolutionized the life sciences since their development as an experimental tool in 2012. Native CRISPR systems act in prokaryotes as an adaptive immune system against invading genetic elements, such as viral DNA. These systems recognize invading nucleic acids, insert segments of the sequence in the host genome, and use these sequences to recognize and destroy the viral element if the cell is invaded again. In recent years, proteins from CRISPR systems, particularly the Cas9 nuclease, have been repurposed for different applications, such as gene editing experiments, large scale genetic screens, and imaging of DNA elements. CRISPR systems have dramatically increased the ease and efficiency of genome engineering, and further investigation and development of these systems is likely to continue for years to come.