The Journal of Young Investigators: An Undergraduate, Peer-Reviewed Science Journal
Volume 19, Issue 17. Nov 2009
Research Archives
Featured Story
Effect of Monday-Thursday Fasting on Working Memory of Adult Human
Abstract
Working memory is the ability in the brain to temporarily hold and process acquired information. To perform memory task, human brain needs sufficient glucose pertaining to its energy demand. Thus, fasting, as a temporary condition of no glucose intake into the body, would logically give negative effect on memory performance. This assumption is supported by the finding of untreated patients of hypoglicemia (low blood glucose) that has syndrome of confusion ...More »
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![[i]Table 1. ACTB Primer and PCR condition details. UPM, universal primer mix, NUP, nested universal primer (SMART RACE, Clontech)[/i]](/articleimages/1662/img0.jpg)
Cloning of beta-actin in early diverging vertebrate little skate (Leucoraja erinacea) for use as a reference gene in real-time PCR
Abstract
β-Actin (ACTB) is a ubiquitously expressed cytoskeletal protein. Involved in cell movement and structure, it is usually expressed at constant levels within the cell. We have successfully cloned and sequenced ACTB cDNA from little skate (Leucoraja erinacea) from the 5’ untranslated region through the poly-adenylated tail. The deduced protein was 374 amino acids in length, with a 99.7% identity to the human ortholog of ACTB. ACTB is highly conserved among vertebrates and is used as ...More »